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+# samtools
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+
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+> Tools for handling high-throughput sequencing (genomics) data.
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+> Used for reading/writing/editing/indexing/viewing of data in SAM/BAM/CRAM format.
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+
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+- Convert a SAM input file to BAM stream and save to file:
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+
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+`samtools view -S -b {{input.sam}} > {{output.bam}}`
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+
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+- Take input from stdin (-) and print the SAM header and any reads overlapping a specific region to stdout:
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+
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+`{{other_command}} | samtools view -h - chromosome:start-end`
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+
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+- Sort file and save to BAM (the output format is automatically determined from the output file's extension):
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+
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+`samtools sort {{input}} -o {{output.bam}}`
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+
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+- Index a sorted BAM file (creates {{sorted_input.bam.bai}}):
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+
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+`samtools index {{sorted_input.bam}}`
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+
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+- Print alignment statistics about a file:
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+
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+`samtools flagstat {{sorted_input}}`
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+
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+- Count alignments to each index (chromosome / contig):
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+
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+`samtools idxstats {{sorted_indexed_input}}`
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+
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+- Merge multiple files:
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+
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+`samtools merge {{output}} {{input_1}} [{{input_2}}...]`
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+
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+- Split input file according to read groups:
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+
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+`samtools split {{merged_input}}`
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